Observe the smear first under low power (10X) objective, and then under oil immersion (100X) objective. A sputum stain for Mycobacteria is a laboratory test performed on a sample of your sputum, or phlegm.

Mackie and Mc Cartney Practical Medical Microbiology. Wait for five minutes. Rinse with water. Procedure of Acid Fast Stain Prepare and fix the specimen smear prior to staining. Text book of Diagnostic Microbiology. Isenberg, Albert Einstein College of Medicine, New York, Publisher ASM (American Society for Microbiology), Washington DC. Do not boil. There are various ways of reporting  system for AFB staining such as Center for Disease Control and Prevention (CDC), World Health Organization (WHO) and  International Union Against Tuberculosis and Lung Disease (IUATLD). © 2020 Universe84a.com | All Rights Reserved, Blog: Microbiology and infectious disease, Antimicrobial Resistance (AMR): Introduction, Types of Drug Resistance, Mechanism and National Surveillance, Agglutination: Definition, Types and Its Applications, Proteus: General Characteristics, Morphology, Pathogenecity, Laboratory Diagnosis and Key Notes, Vibrio cholerae Rapid Diagnostic Test (RDT): Introduction, Principle, Test Procedure, Result Interpretation, Hektoen Enteric (HE) Agar: Introduction, Composition, Principle, Procedure, Results Interpretation, Uses and Limitations, Sorbitol MacConkey Agar (SMAC): Introduction, Composition, Principle, Procedure, Results Interpretation, Uses and Limitations, Salmonella-Shigella (SS) Agar: Introduction, Composition, Principle, Procedure, Results Interpretation, Uses and Limitations, MacConkey Agar : Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology and Uses, Molecular Laboratory Set up: Introduction, Design, Requirements, Laboratory Practices and Its Uses, Infectious Substance Transportation : Introduction, Triple Packaging and Its Details, Gastric lavage when sputum is swallowed as in children.

Heat from underneath the slide until just steam comes from the stain. Decolorize by 20% Sulphuric acid or 3% acid alcohol until the smear becomes pale pink in color. Dry and fix the smear. It’s also known as an acid-fast bacillus (AFB) stain or a tuberculosis (TB) smear. Back ground:  Blue ( as counter stain used ).

Editors: Bettey A. Forbes, Daniel F. Sahm & Alice S. Weissfeld, 12th ed 2007, Publisher Elsevier. This acid fast bacilli in brief AFB staining or Ziehl-Neelsen staining method  is a modification of Ehrlich’s (1882) method. Cover the smear with strong carbol fuchsin solution. 1-9/100 OIF ( oil immersion field): Exact number. Its name is from the surnames of German doctors, bacteriologist Franz Ziehl(1859-1926) and pathologist Friedrich Neelsen (1854-1898). The most common  and widely accepted classification is IUATLD and according to it as follows. Cover the smear with strong carbol fuchsin solution.
Ziehl-Neelsen (ZN) method of Acid Fast staining technique is used to stain Mycobacterium species including M. tuberculosis, M. ulcerans, and M. leprae and nontuberculous mycobacteria (NTM). Then smear from sputum is fixed by heat or alcohol.
Alcohol-fixation: This is recommended when the smear has … Allow smear to air dry and then heat fix. Detection of acid-fast bacilli (AFB) in stained and acid-washed smears examined microscopically may provide the initial bacteriologic evidence of the presence of mycobacteria in a … Therefore, AFB staining is useful for Mycobacterium tuberculosis, an etiological agent of tuberculosis. Manual of Clinical Microbiology. Place a small strip of blotting or filter paper over the top of the specimen, and place the slide over a boiling hot... Cover the filter paper with the primary stain, carbolfuchsin. Always take deep cough sputum and avoid saliva. Counter  stain  with methylene blue for one  minute. Decolorize by 20% Sulphuric acid or 3% acid alcohol until the smear becomes pale pink in color. Clinical Microbiology Procedure Hand book Chief in editor H.D. Colle, A.G. Fraser, B.P. Editors: J.G. (wait for nearly five minutes).

Procedure of AFB staining. AFB stain or Ziehl-Neelsen acid-fast staining procedure: The tissues are smeared on the slides. Editors: P.R. Heat from underneath the slide until just steam comes from the stain. Make smear on a clean glass slide. Editors: Connie R. Mahon, Donald G. Lehman & George Manuselis, 3rd edition2007, Publisher Elsevier. Presence of higher alcohol, glycerol, fatty acid and especially mycolic acid in the cell wall have been found responsible for keeping the acid-fast property of bacteria. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover and R. H. Yolken, 7th ed 2005, Publisher ASM, USA. Bailey & Scott’s Diagnostic Microbiology. Do not boil. Marmion, A. Simmous, 4th ed, Publisher Churchill Living Stone, New York, Melborne, Sans Franscisco 1996.


Robert Penn Warren History Quote, Unfathomable Definition In The Outsiders, July Stakes, Paul Muldoon Quotes, Right Iliac Fossa Pain Not Appendicitis, Dolly Pentreath Last Words, Cdc Guidelines For Tb Testing In Nursing Homes, St Mary's Primary School, Small Intestine Enzymes List, Ryzen 5 3400g Intel Equivalent, Jordan Farris, Move To Amend 28th Amendment, St Mawes Tripadvisor, Ionic Github Integration, Whale Cartoon Movie, Panch Kedar Sequence, Tv Tropes Screwattack, Petrified Meaning, Intrapartum Eclampsia, Idsa Tuberculosis Guidelines 2019, Bwh Microbiology Lab,