Serial cryostat sections of the aortic arch were prepared using a Lab‐Tek tissue processor (Leica, Solms, Germany).The aortic sections were stained with CD31 (Abcam) and DAPI. Images were acquired on an upright Leica SP8 confocal microscope. TLRs) capture antigens and quickly leave the tissue. Alzheimer's disease: Innate immunity gone awry?. Digestion mixtures were then treated with 0.1 M EDTA (Sigma, St Louis, MO) for 5 minutes and were centrifuged to remove tissue debris, and cells were washed in RPMI 1640 (Irvine Scientific, Santa Ana, CA) containing 2% fetal calf serum (FCS; Omega Scientific, Tarazana, CA). Dual-staining demonstrated that DEC-205 was primarily expressed on the CD8α+ subset in spleen and lymph nodes, consistent with its established expression on lymphoid DCs. The ultrastructure and size distribution of exosomes were analysed by transmission electron microscopy and Nanosight (Malvern, Malvern, UK) respectively.
2015 Mar 1;194(5):2369-79. doi: 10.4049/jimmunol.1402412. Electron microscopic analysis revealed a typical size of 30–100 nm in diameter (Fig. Effect of recombinant human granulocyte-macrophage colony-stimulating factor on myelopoiesis in patients with refractory metastatic carcinoma. Vésicules extracellulaires, biomarqueurs et bioeffecteurs du syndrome métabolique. ***P<0.001. Roles of Exosomes Derived From Immune Cells in Cardiovascular Diseases. This site needs JavaScript to work properly. Interleukin-12 and Flt3 ligand differentially promote dendropoiesis in vivo. Extracellular Vesicles: How Drug and Pathology Interfere With Their Biogenesis and Function. Clipboard, Search History, and several other advanced features are temporarily unavailable. Despite these independent effects of GM-CSF, there is evidence that IL-4 still plays an important synergistic role in generating mouse DCs. Development of CD8α-positive dendritic cells from a common myeloid progenitor. After 24 hrs of activation by PBS or LPS, the culture medium was replaced completely.
Further studies are indicated to determine the types of precursors that respond to rGM-CSF/rIL-4 and the impact of this therapy on immune responsiveness mediated by different DC subpopulations in vivo. 4A and B). Please enable it to take advantage of the complete set of features! Saroj K. Basak, Airi Harui, Marina Stolina, Sherven Sharma, Kohnosuke Mitani, Steven M. Dubinett, Michael D. Roth; Increased dendritic cell number and function following continuous in vivo infusion of granulocyte macrophage–colony-stimulating factor and interleukin-4. Jiao Q, Liu C, Li W, Li W, Fang F, Qian Q, Zhang X. Clin Exp Immunol.


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