If you are unable to produce sputum, a bronchoscope may be used to collect fluid during a procedure called a bronchoscopy. Depending on symptoms, urine, an aspirate from the site of suspected infection, cerebrospinal fluid (CSF) , other body fluids , or biopsied tissue samples may be collected for AFB testing. [ ] C&S TEST: Collection 1. A sputum culture requires minimal effort on your part. A technician may visually check the color of the sputum that can give an idea about the type of the infection. Specimens must be collected at the right time, using the correct technique and equipment, and be delivered to the laboratory in a timely manner (Dougherty and Lister, 2015). It’s also known as an acid-fast bacillus (AFB) stain or a tuberculosis (TB) smear. In children, gastric washings/ aspirates may be collected.

Sputum induction is a procedure used for patients who have trouble producing sputum spontaneously. The patient inhales nebulised hypertonic saline solution, which liquefies airway secretions, promotes coughing and allows expectoration of respiratory secretions. Ideally, the sample should be collected from a deep cough early in the morning. A sputum stain for Mycobacteria is a laboratory test performed on a sample of your sputum, or phlegm. Sputum is material brought up from the lungs after a deep cough. AFB tests are usually ordered for people with symptoms of active TB. It is different from spit or saliva. Obtaining a specimen involves collecting tissue or fluids for laboratory analysis or near-patient testing, and may be a first step in determining a diagnosis and treatment (Dougherty and Lister, 2015). [ 1] S

The tests look for the presence of AFB bacteria in your sputum. You might need to rinse your mouth with water first, and your doctor might ask you to skip a meal or stop taking any bacteria-killing antibiotics you’ve been given before the test.

Title: Sputum - CS - TB - AFB Doc.#38116 Ver 1.0 Page 1 of 1 Patient Instructions Sputum – C&S or TB/AFB Your health care provider has requested a sputum specimen(s) for the test(s) marked below [x]. In some cases, more than one sample may be needed. You’ll be asked to cough deeply to bring up the sputum … The sputum that is coughed up by the patient is collected in a sterilized container for the necessary testing. Sputum is a thick mucus that is coughed up from the lungs. In order to produce a meaningful laboratory test result, follow these instructions exactly. How is the sputum test performed? Box 1 provides a reminder of the general principle of speci… You simply need to provide the sample for the lab to test.

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