If using a regular sterile suction catheter, remove gloves, perform hand hygiene, don sterile gloves, and avoid contaminating the outside of the container. 47 Sputum is induced by hypertonic saline inhalation and sputum plugs are isolated from the sample, diluted 1/10 in 0.1% dithiothreitol (DTT), and mixed at room temperature for 30 min to disperse the cells. Collect the expectorated sputum in a leak-proof sterile container. The following assay allows the measurement in sputum samples of total ECP levels and of ECP released by eosinophil degranulation. If using a sleeved suction catheter, remove the suctioning tubing from the end of the catheter and connect it to the sputum trap. Steam inhalation can also be used at times. Store specimens at 2-8°C for up to 72 hours after collection. If someone cannot cough up any sputum, they may be asked to inhale a sterile saline or glycerine solution to loosen up the phlegm in the lungs. Have the patient rinse the mouth with water and then expectorate deep cough sputum directly into a sterile, leak-proof, screw-cap collection cup or sterile dry container. Educate the patient about the difference between sputum and oral secretions (saliva). Storage. ⇒ Streak the Sputum specimen on the Blood agar plate using a well sterilized inoculating loop. II. Sterile swab Tray for assembling sputum collection tools Clean water for oral rinse Plague Rapid Diagnostic Test (if available) Durable marker for writing on laboratory samples Step 1b: Assemble equipment for preventing infections For hand hygiene use Connect the suction tubing to the adapter on the sputum trap. ⇒ Inoculate Thioglycollate tube with small loopful of a selected portion of the sputum. Refrigerate the container until processing takes place. Sterility is important for culture results. ⇒ Place the inoculated blood agar plate in a candle jar (Anaerobic condition). Sputum. A sputum Gram’s stain is a laboratory test that allows your doctor to diagnose a bacterial infection in your respiratory tract.


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